![]() However, these culture systems are limited in their production capacity and are not easily amenable to process‐control strategies. These culture systems maintain a balance between allowing ES cell aggregation necessary for EB formation and preventing EB agglomeration for efficient cell growth and differentiation. Standard methods of EB formation include hanging drop, liquid suspension, and methylcellulose culture. Presently, all human and most mouse ES cell lines require aggregation of multiple ES cells to efficiently initiate EB formation. EB differentiation has been shown to recapitulate aspects of early embryogenesis, including the formation of a complex three‐dimensional architecture wherein cell‐cell and cell‐matrix interactions are thought to support the development of the three embryonic germ layers and their derivatives. Consequently, the most robust method for generating most differentiated cell types is through the embryoid body (EB) system where ES cells spontaneously differentiate as tissue‐like spheroids in suspension culture. In most cases, the knowledge to precisely control mouse or human ES cell fate decisions is lacking. (2001) described culture conditions that exclusively permit the formation of neural progenitor cells from mouse ES cells, albeit at very low cell frequency. In an ideal scenario, differentiation of ES cells could be directed to a pure population of the desired cell type. This can be accomplished by increasing the scale of cell production and by optimizing culture conditions for the generation of target cells. While ES‐derived cells have tremendous potential in many experimental and therapeutic applications, their utility, and indeed investigations into the scope of their utility, is dependent on the availability of relevant cell quantities. ES cells can therefore be considered a renewable source of useful cell types such as cardiomyocytes, insulin‐secreting cells, dopaminergic neurons, and hematopoietic progenitors -cells that have proven difficult to expand in vitro. Stem cell culture, Encapsulation, Embryonic stem cells, Embryoid body, Scalable I ntroductionĮmbryonic stem (ES) cells are pluripotent cells capable of extensive proliferation while maintaining their potential to differentiate into any cell type in the body.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |